CAV1 Knockout cell line (MCF7)

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CAV1 Knockout cell line (MCF7) Order

CAV1 Knockout cell line (MCF7)
Catalog#
YKO-H254
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CAV1 Knockout cell line (MCF7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CAV1 Knockout cell line (MCF7)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
CAV1
Gene ID
857
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
87%MEM+10%FBS+1%Glutamax+1%SodiumPyruvate+1%NEAA
Accession
CVCL_E0YE(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript CAV1-201)
Validation: PCR+Sanger Sequencing
CAV1 Knockout cell line (MCF7) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:MCF7		 STR Info (Cell bank)
Cell Line:MCF-7
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11111111
D7S820 8989
D16S539 11121112
VWA 14151415
TH01 6666
AMEL XXXX
TPOX 912912
CSF1PO 10101010
D12S391 1820
FGA 2325
D2S1338 2123
D21S11 3030
D18S51 1414
D8S1179 1014
D3S1358 1616
D6S1043 1218
PENTAE 712
D19S433 1313
PENTAD 1212
D1S1656 1115.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CAV1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MCF7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CAV1 gene knockout CAV1 Knockout cell line (MCF7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
CAV1 Knockout cell line (MCF7)
CAV1 Knockout cell line (MCF7)
Catalog#
YKO-H254
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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